If you play basic for thousands of hands, yes, playing perfectly will make a difference (and that is why it is so exhausting to try to make it work at a substantial profit).
The chart doesn't show the odds underlying the basic rules. One thing I get a kick out of is when a 'perfect player' gets bent out of shape at someone making one of those choices where basic strategy says to do something, but the differential is only a couple thousandths of a % between hitting or staying. One reason for this is we are likely to just get annoyed in general when we see somebody doing things 'wrong.' So when it affects us, we get annoyed even more, and it sticks in the memory more strongly. It just seems to be whenever I get up to a decent size bet is when a player will deviate from basic strategy and seem to mess up that particular hand and I lose
You are absolutely correct that in the grand scheme of things it all balances out.
